Acetaminophen Toxicity Explained Stages, Diagnosis & N Acetylcysteine Treatment CanadaQBank Liver injury induced by paracetamol and challenges associated with intentional and unintentional use Acetaminophen causes neurodevelopmental injury in susceptible babies and children: no valid rationale for controversy Acetaminophen cytotoxicity in HepG2 cells is associated with a decoupling of glycolysis from the TCA cycle, loss of NADPH production, and suppression of anabolism Archives of Toxicology Springer Nature Link Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.