Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione You Are The Healer Tylenol's Impact on Glutathione and Liver Damage The mechanism of acetaminophen action! The mechanism of action of acetaminophen (paracetamol), a common analgesic, is not yet clear. The study shows that acetaminophen counterintuitively inhibits diacylglycerol lipase (DAGL), but not Full article: Paracetamol (acetaminophen) overdose and hepatotoxicity: mechanism, treatment, prevention measures, and estimates of burden of disease