Acetaminophen Depletes Glutathione Essential Formulas Tylenol Avoid or Not to Avoid? Acetaminophen (Tylenol) can deplete glutathione, your body's most important detox antioxidant. This matters even more if your child has an MTHFR gene mutation, which Hepsin as a potential therapeutic target for alleviating acetaminophen induced hepatotoxicity via gap junction regulation and oxidative stress modulation Cell Biology and Toxicology Springer Nature Link In vivo upstream factors of mouse hepatotoxic mechanism with sustained hepatic glutathione depletion: Acetaminophen metabolite erythrocyte adducts and splenic macrophage generated reactive oxygen species ScienceDirect Frontiers Liquid Chromatography Tandem Mass Spectrometry Analysis of Acetaminophen Covalent Binding to Glutathione S Transferases