Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Tylenol And Glutathione Invigor Medical Tylenol Avoid or Not to Avoid? Acetaminophen (Tylenol) can deplete glutathione, your body's most important detox antioxidant. This matters even more if your child has an MTHFR gene mutation, which Sex differences in glutathione metabolism and acetaminophen toxicity